Composite

Part:BBa_K1217003:Design

Designed by: Ma Tsz Shan   Group: iGEM13_Hong_Kong_HKU   (2013-09-15)


pT7-ppk1(K.oralis)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 309


Design Notes

We choose to amplify ppk1 gene from Kingella oralis (ATCC 51147) since the sequence does not contain any of the illegal restriction sites.


Source

The coding gene ppk1(E.C. 2.7.4.1) is amplified from Kingella oralis (ATCC 51147) genomic DNA.

References